The olfactory system has the unusual capacity to generate new neurons throughout the lifetime of an organism. This allows to study stem cell function and the regulation of neurogenesis in the developing and adult olfactory system. We investigate how newly generated olfactory neurons integrate into the existing olfactory circuitry. Thereby, we are particularly interested in how axons of olfactory receptor neurons find their way to the correct target areas in the olfactory bulb, the wiring of the network, and how the formation of new synapses in glomeruli is regulated.
We investigate general functional properties of the sense of smell. Fluorescent microscopy and electrophysiological tools are applied to measure cellular activity in both the olfactory epithelium and the forebrain. This allows to investigate the cellular machinery necessary for odorant detection & modulation and the general principles of odor mapping & processing in the brain. We also would like to understand why during evolution, olfactory systems show a tendency to seggregate into distinct, specialized subsystems.
The olfactory system with its unique strengths is a useful tool to study the different steps in the maturation and regeneration of neurons on a molecular, cellular and functional level. We aim to understand the mechanisms that regulate olfactory neurogenesis during normal tissue maintenance and under repair conditions. The knowledge created as the result of basic research is likely to assist medical treatment and the development of new therapies targeted at replacement of lost neurons in neurodegenerative disorders or following nervous system injury.
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Offner T, Daume D, Weiss L, Hassenklöver L, Manzini I
Whole-brain calcium imaging in larval Xenopus.
Cold Spring Harb. Protoc., in press.
Richter K, Ogiemwonyi-Schaefer R, Wilker S, Chaveiro AI, Agné A, Hecker M, Reichert M, Amati A-L, Schlüter K-D, Manzini I, Schmalzing G, McIntosh JM, Padberg W, Grau V, Hecker A
Amyloid beta peptide (Aβ 1-42) reverses the cholinergic control of monocytic IL-1β release.
J. Clin. Med., 9(9):E2887.
Weiss L, Jungblut LD, Pozzi AG, O'Connell LA, Hassenklöver T, Manzini I
Conservation of glomerular organization in the main olfactory bulb of anuran larvae.
Front. Neuroanat., 14:44.
Gerdes C, Waal N, Offner T, Fornasiero EF, Wender N, Verbarg H, Manzini I, Trenkwalder C, Mollenhauer BB, Strohaeker T, Zweckstetter M, Becker S, Rizzoli SO, Basmanav B, Opazo F
A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol.
Nat. Commun., 11:2729.
Weiss L, Jungblut LD, Pozzi AG, O'Connell LA, Zielinski BS, Hassenklöver T, Manzini I
Multi-glomerular projection of single olfactory receptor neurons is conserved among amphibians.
J. Comp. Neurol., 28(13):2239-2253.
Demirler MC, Sakizli U, Bali B, Kocagöz Y, Eski SE, Ergönen A, Alkiraz AS, Bayramli X, Hassenklöver T, Manzini I, Fuss SH
Purinergic signalling selectively modulates maintenance but not repair neurogenesis in the zebrafish olfactory epithelium.
FEBS J., 287(13):2699-2722.
Wichmann L, Dulai JS, Marles-Wright J, Maxeiner S, Szczesniak PP, Manzini I, Althaus M
An extracellular acidic cleft confers profound H+-sensitivity to epithelial sodium channels containing the δ-subunit in Xenopus laevis.
J. Biol. Chem., 294(33):12507-12520.
Zakrzewicz A, Richter K, Zakrzewicz D, Siebers K, Damm J, Agné A, Hecker A, McIntosh JM, Chamulitrat W, Krasteva-Christ G, Manzini I, Tikkanen R, Padberg W, Janciauskiene S, Grau V
SLPI inhibits ATP-mediated maturation of IL-1β in human monocytic leukocytes: A novel function of an old player.
Front. Immunol., 10:664.
Weiss L, Offner T, Hassenklöver T, Manzini I
Dye electroporation and imaging of calcium signaling in Xenopus nervous system.
Methods Mol. Biol., 1865:217-231.
Wichmann L, Vowinkel KS, Perniss A, Manzini I, Althaus M
Incorporation of the delta-subunit into the epithelial sodium channel (ENaC) generates protease-resistant ENaCs in Xenopus laevis.
J. Biol. Chem., 293(18):6647-6658.
Hiller SD, Heldmann S, Richter K, Jurastow I, Küllmar M, Hecker A, Wilker S, Fuchs-Moll G, Manzini I, Schmalzing G, Kummer W, Padberg W, McIntosh JM, Damm J, Zakrzewicz A, Grau V
β-Nicotinamide adenine dinucleotide (β-NAD) inhibits ATP-dependent IL-1β release from human monocytic cells.
Int. J. Mol. Sci., 19(4):1126.
Hawkins SJ, Weiss L, Offner T, Dittrich K, Hassenklöver T, Manzini I
Functional reintegration of sensory neurons and transitional dendritic reduction of mitral/tufted cells during injury-induced recovery of the larval Xenopus olfactory circuit.
Front. Cell. Neurosci., 11:380.
Backhaus S, Zakrzewicz A, Richter K, Damm J, Wilker S, Fuchs-Moll G, Kuellmar M, Hecker A, Manzini I, Ruppert C, McIntosh JM, Padberg W, Grau V
Surfactant inhibits ATP-induced release of interleukin-1β via nicotinic acetylcholine receptors.
J. Lipid Res., 58(6):1055-1066.
Syed AS, Sansone A, Hassenklöver T, Manzini I, Korsching SI
Coordinated shift of olfactory amino acid responses and V2R expression to an amphibian water nose during metamorphosis.
Cell. Mol. Life Sci., 74(9):1711-1719.